Membrane topology of the GltS Na+/glutamate permease of Escherichia coli.
نویسندگان
چکیده
The GltS Na+/glutamate permease of Escherichia coli is the most extensively studied member of the ESS family of bacterial glutamate:Na+ symporters. This paper presents the membrane topology analysis of the GltS with translational alkaline phosphatase and beta-galactosidase gene fusions generated by TnphoA, nested deletions and targeted fusions. The topology model suggested by the translational fusion technique is compared with the MemGen model and discussed in detail.
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ورودعنوان ژورنال:
- FEMS microbiology letters
دوره 275 1 شماره
صفحات -
تاریخ انتشار 2007